解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in Nematostella, a representative pre-bilaterian animal where PGCs arise as paired ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54573
更新日期:2020-09-24 00:00:00
abstract::Experiments on mitochondrial DNA in worms highlight that cheating does not always pay off. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.62222
更新日期:2020-09-22 00:00:00
abstract::Prion diseases are caused by PrPSc, a self-replicating pathologically misfolded protein that exerts toxicity predominantly in the brain. The administration of PrPSc causes a robust, reproducible and specific disease manifestation. Here, we have applied a combination of translating ribosome affinity purification and ri...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.62911
更新日期:2020-09-22 00:00:00
abstract::KdpFABC is an ATP-dependent K+ pump that ensures bacterial survival in K+-deficient environments. Whereas transcriptional activation of kdpFABC expression is well studied, a mechanism for down-regulation when K+ levels are restored has not been described. Here, we show that KdpFABC is inhibited when cells return to a ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55480
更新日期:2020-09-21 00:00:00
abstract::Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haph...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55308
更新日期:2020-09-21 00:00:00
abstract::The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli and receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling and activity of neurons expressing CaMKIIα (a marker for glutamatergic principal cells) in mice during...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57335
更新日期:2020-09-18 00:00:00
abstract::Cells use molecular circuits to interpret and respond to extracellular cues, such as hormones and cytokines, which are often released in a temporally varying fashion. In this study, we combine microfluidics, time-lapse microscopy, and computational modeling to investigate how the type I interferon (IFN)-responsive reg...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58825
更新日期:2020-09-18 00:00:00
abstract::The insular cortex (IC) plays key roles in emotional and regulatory brain functions and is affected across psychiatric diseases. However, the brain-wide connections of the mouse IC have not been comprehensively mapped. Here, we traced the whole-brain inputs and outputs of the mouse IC across its rostro-caudal extent. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55585
更新日期:2020-09-17 00:00:00
abstract::Phenazines are natural bacterial antibiotics that can protect crops from disease. However, for most crops it is unknown which producers and specific phenazines are ecologically relevant, and whether phenazine biodegradation can counter their effects. To better understand their ecology, we developed and environmentally...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59726
更新日期:2020-09-15 00:00:00
abstract::During mitosis chromosomes reorganise into highly compact, rod-shaped forms, thought to consist of consecutive chromatin loops around a central protein scaffold. Condensin complexes are involved in chromatin compaction, but the contribution of other chromatin proteins, DNA sequence and histone modifications is less un...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57212
更新日期:2020-09-11 00:00:00
abstract::In Arabidopsis thaliana, winter is registered during vernalization through the temperature-dependent repression and epigenetic silencing of floral repressor FLOWERING LOCUS C (FLC). Natural Arabidopsis accessions show considerable variation in vernalization. However, which aspect of the FLC repression mechanism is mos...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57671
更新日期:2020-09-09 00:00:00
abstract::Drug treatment of 3D cancer spheroids more accurately reflects in vivo therapeutic responses compared to adherent culture studies. In EGFR-mutated lung adenocarcinoma, EGFR-TKIs show enhanced efficacy in spheroid cultures. Simultaneous inhibition of multiple parallel RTKs further enhances EGFR-TKI effectiveness. We sh...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58204
更新日期:2020-09-08 00:00:00
abstract::Jellyfish, with their tetraradial symmetry, offer a novel paradigm for addressing patterning mechanisms during regeneration. Here we show that an interplay between mechanical forces, cell migration and proliferation allows jellyfish fragments to regain shape and functionality rapidly, notably by efficient restoration ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54868
更新日期:2020-09-07 00:00:00
abstract::The asymmetric outer membrane (OM) of Gram-negative bacteria functions as a selective permeability barrier to the environment. Perturbations to OM lipid asymmetry sensitize the cell to antibiotics. As such, mechanisms involved in lipid asymmetry are fundamental to our understanding of OM lipid homeostasis. One such me...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56571
更新日期:2020-09-03 00:00:00
abstract::Seasonal influenza virus A/H3N2 is a major cause of death globally. Vaccination remains the most effective preventative. Rapid mutation of hemagglutinin allows viruses to escape adaptive immunity. This antigenic drift necessitates regular vaccine updates. Effective vaccine strains need to represent H3N2 populations ci...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60067
更新日期:2020-09-02 00:00:00
abstract::Successful fertilization in angiosperms depends on the proper trajectory of pollen tubes through the pistil tissues to reach the ovules. Pollen tubes first grow within the cell wall of the papilla cells, applying pressure to the cell. Mechanical forces are known to play a major role in plant cell shape by controlling ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57282
更新日期:2020-09-01 00:00:00
abstract::The importance of cultural processes to behavioural diversity in our closest living relatives is central to revealing the evolutionary origins of human culture. However, the bonobo is often overlooked as a candidate model. Further, a prominent critique to many examples of proposed animal cultures is premature exclusio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59191
更新日期:2020-09-01 00:00:00
abstract::Yeast cells undergoing the diauxic response show a striking upstream shift in poly(A) site utilization, with increased use of ORF-proximal poly(A) sites resulting in shorter 3' mRNA isoforms for most genes. This altered poly(A) pattern is extremely similar to that observed in cells containing Pol II derivatives with s...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59810
更新日期:2020-08-26 00:00:00
abstract::Breast cancer stem cells (BCSCs) contribute to intra-tumoral heterogeneity and therapeutic resistance. However, the binary concept of universal BCSCs co-existing with bulk tumor cells is over-simplified. Through single-cell RNA-sequencing, we found that Neu, PyMT and BRCA1-null mammary tumors each corresponded to a sp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58810
更新日期:2020-08-25 00:00:00
abstract::Ageing is characterised by cellular senescence, leading to imbalanced tissue maintenance, cell death and compromised organ function. This is first observed in the thymus, the primary lymphoid organ that generates and selects T cells. However, the molecular and cellular mechanisms underpinning these ageing processes re...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56221
更新日期:2020-08-25 00:00:00
abstract::We tested the proposal that the C-terminal domain (CTD) of the AMPAR subunit GluA1 is required for LTP. We found that a knock-in mouse lacking the CTD of GluA1 expresses normal LTP and spatial memory, assayed by the Morris water maze. Our results support a model in which LTP generates synaptic slots, which capture pas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58042
更新日期:2020-08-24 00:00:00
abstract::The β-catenin transcriptional coregulator is involved in various biological and pathological processes; however, its requirements in hematopoietic cells remain controversial. We re-targeted the Ctnnb1 gene locus to generate a true β-catenin-null mutant mouse strain. Ablation of β-catenin alone, or in combination with ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55360
更新日期:2020-08-21 00:00:00
abstract::Dopamine and striatal dysfunctions play a key role in the pathophysiology of Parkinson's disease (PD) and Dystonia, but our understanding of the changes in the discharge rate and pattern of striatal projection neurons (SPNs) remains limited. Here, we recorded and examined multi-unit signals from the striatum of PD and...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57445
更新日期:2020-08-19 00:00:00
abstract::We aimed to study time trends and levels of mean total cholesterol and lipid fractions, and dyslipidaemias prevalence in Latin America and the Caribbean (LAC). Systematic-review and meta-analysis of population-based studies in which lipid (total cholesterol [TC; 86 studies; 168,553 people], HDL-Cholesterol [HDL-C; 84 ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57980
更新日期:2020-08-18 00:00:00
abstract::The eukaryotic replisome assembles around the CMG helicase, which stably associates with DNA replication forks throughout elongation. When replication terminates, CMG is ubiquitylated on its Mcm7 subunit and disassembled by the Cdc48/p97 ATPase. Until now, the regulation that restricts CMG ubiquitylation to terminatio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60371
更新日期:2020-08-17 00:00:00
abstract::Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 amino acids. Here we present high-resolution structures of several DHBV caps...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57277
更新日期:2020-08-14 00:00:00
abstract::Plasticity can put evolution on repeat if development causes species to generate similar morphologies in similar environments. Anolis lizards offer the opportunity to put this role of developmental plasticity to the test. Following colonization of the four Greater Antillean islands, Anolis lizards independently and re...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57468
更新日期:2020-08-13 00:00:00
abstract::Down syndrome (DS) results in various degrees of cognitive deficits. In DS mouse models, recovery of behavioral and neurophysiological deficits using GABAAR antagonists led to hypothesize an excessive activity of inhibitory circuits in this condition. Nonetheless, whether over-inhibition is present in DS and whether t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58731
更新日期:2020-08-12 00:00:00
abstract::The representation of position in the mammalian brain is distributed across multiple neural populations. Grid cell modules in the medial entorhinal cortex (MEC) express activity patterns that span a low-dimensional manifold which remains stable across different environments. In contrast, the activity patterns of hippo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56894
更新日期:2020-08-11 00:00:00
abstract::The dynamic tyrosination-detyrosination cycle of α-tubulin regulates microtubule functions. Perturbation of this cycle impairs mitosis, neural physiology, and cardiomyocyte contraction. The carboxypeptidases vasohibins 1 and 2 (VASH1 and VASH2), in complex with the small vasohibin-binding protein (SVBP), mediate α-tub...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58157
更新日期:2020-08-10 00:00:00
abstract::Mitochondria are dynamic organelles that must precisely control their protein composition according to cellular energy demand. Although nuclear-encoded mRNAs can be localized to the mitochondrial surface, the importance of this localization is unclear. As yeast switch to respiratory metabolism, there is an increase in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57814
更新日期:2020-08-07 00:00:00
abstract::A strain of budding yeast that contains one large chromosome reveals how the telomere capping complex CST maintains linear but not circular chromosomes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60150
更新日期:2020-08-05 00:00:00
abstract::The cellular architecture of the ventral tegmental area (VTA), the main hub of the brain reward system, remains only partially characterized. To extend the characterization to inhibitory neurons, we have identified three distinct subtypes of somatostatin (Sst)-expressing neurons in the mouse VTA. These neurons differ ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59328
更新日期:2020-08-04 00:00:00
abstract::Negative feedback regulation, that is the ability of a gene to repress its own synthesis, is the most abundant regulatory motif known to biology. Frequently reported for transcriptional regulators, negative feedback control relies on binding of a transcription factor to its own promoter. Here, we report a novel mechan...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58836
更新日期:2020-08-03 00:00:00
abstract::The NuRD complex subunit CHD4 is essential for fusion-positive rhabdomyosarcoma (FP-RMS) survival, but the mechanisms underlying this dependency are not understood. Here, a NuRD-specific CRISPR screen demonstrates that FP-RMS is particularly sensitive to CHD4 amongst the NuRD members. Mechanistically, NuRD complex con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54993
更新日期:2020-08-03 00:00:00
abstract::High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is unclear. We examined seven high-altitude ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56259
更新日期:2020-07-30 00:00:00
abstract::Conventional Ly6Chi monocytes have developmental plasticity for a spectrum of differentiated phagocytes. Here we show, using conditional deletion strategies in a mouse model of Toll-like receptor (TLR) 7-induced inflammation, that the spectrum of developmental cell fates of Ly6Chi monocytes, and the resultant inflamma...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57007
更新日期:2020-07-29 00:00:00
abstract::Pioneer factors such as Zelda (Zld) help initiate zygotic transcription in Drosophila early embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental pattern...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.59610
更新日期:2020-07-23 00:00:00
abstract::Early life adversity (ELA) is associated with increased risk for stress-related disorders later in life. The link between ELA and risk for psychopathology is well established but the developmental mechanisms remain unclear. Using a mouse model of resource insecurity, limited bedding (LB), we tested the effects of LB o...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55263
更新日期:2020-07-21 00:00:00
abstract::Lissencephaly ('smooth brain') is a severe brain disease associated with numerous symptoms, including cognitive impairment, and shortened lifespan. The main causative gene of this disease - lissencephaly-1 (LIS1) - has been a focus of intense scrutiny since its first identification almost 30 years ago. LIS1 is a criti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59737
更新日期:2020-07-21 00:00:00